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American Society of Microbiology ABMM Sample Questions:

1. The human virome, the collection of viruses that reside in and on the human body, is increasinglyrecognized for its role in health and disease. Which of the following statements regarding the human virome is MOST accurate?

A) The composition of the human virome is relatively stable over time and is not influenced by environmental factors.
B) The human virome is largely independent of the host's immune status.
C) The human virome is primarily composed of pathogenic viruses that cause acute infections.
D) Bacteriophages, viruses that infect bacteria, are a significant component of the human gut virome.

2. A research laboratory is investigating the mechanism of action of a novel antiviral compound that inhibits the replication of a specific RNA virus. They find that the compound binds directly to the viral RNA-dependent RNA polymerase, preventing its elongation activity. This mechanism is most similar to that of:

A) Acyclovir
B) Ribavirin
C) Interferon-beta
D) Oseltamivir

3. A diagnostic laboratory is validating a new multiplex PCR panel for respiratory pathogens. According to CLIA regulations, which of the following parameters must be established by the laboratory performing the validation for this qualitative nucleic acid amplification test?

A) Accuracy, Precision, Reportable Range, Analytical Sensitivity
B) Precision, Reportable Range, Analytical Specificity, Reference Interval
C) Accuracy, Precision, Analytical Sensitivity, Analytical Specificity (including cross-reactivity)
D) Accuracy, Precision, Clinical Sensitivity, Clinical Specificity

4. A traveler returning from Southeast Asia presents with fever, headache, and a maculopapular rash that started on the trunk and spread to the extremities, including the palms and soles. Serological testing is positive for Orientia tsutsugamushi. The vector responsible for transmitting this organism is a:

A) Mosquito
B) Flea
C) Tick
D) Mite (chigger)

5. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?

A) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.
B) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.
D) Positive controls for each target organism and a negative control without any template DNA.

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